Tag: Research

  • Study reveals novel approach to promote efficient uptake of ASOs into cancer cells

    Study reveals novel approach to promote efficient uptake of ASOs into cancer cells

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    Antisense oligonucleotides (ASOs) are next-generation drugs that can treat disease by blocking the transfer of harmful messages from our genes. In people with cancer, ASOs have the potential to block messages that encourage the growth and spread of the tumor. However, ASOs aren’t used for treating cancer yet. They must first get delivered inside cancer cells, but the cancer cells won’t let them in.

    Finding an effective ASO delivery system is a major challenge. Cancer cells have gatekeeper molecules that stop unwanted substances from entering. Although investigators have tried many ways of getting ASOs past the gatekeepers, success has been limited.

    Now, in a study recently published in the journal Nucleic Acids Research, researchers from Osaka University have discovered a way to deliver ASOs to their targets inside cancer cells. The team synthesized a new compound, named L687, which opens specific calcium permeable channels on the surface of cancer cells. When the calcium flows into cells through the open channels it tells the cells to let in the ASOs.

    We discovered that we could selectively activate the TRPC3/C6 calcium permeable channels with the activator L687. We then found that combination treatment with L687 and ASO promoted efficient uptake of ASO into cancer cells during laboratory tests and tumor cells inside the mouse. As a result, target gene activity was suppressed and ASO efficacy was enhanced.”


    Hiroto Kohashi, Lead Author

    Until now, ASOs have mainly been used to treat incurable diseases and had to be delivered into the liver or spinal fluid. According to the Osaka team’s research, L687 is an effective drug delivery system that may extend the benefits of ASO treatment to other parts of the body.

    “We hope that the results of our research will lead to significant progress in the development and delivery of ASOs and similar gene-targeting drugs for treating cancer,” says senior author Masahito Shimojo.

    The team believes that L687 could be a particularly effective way of delivering ASO therapy to lung or prostate cancers. These cancers have many TRPC3/C6 calcium permeable channels that can be opened by L687, potentially revealing new targets for these next-generation therapies.

    Source:

    Journal reference:

    Kohashi, H., et al. (2024) A novel transient receptor potential C3/C6 selective activator induces the cellular uptake of antisense oligonucleotides. Nucleic Acids Research. doi.org/10.1093/nar/gkae245.

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  • Study reveals human gut plasmid with biomarker potential

    Study reveals human gut plasmid with biomarker potential

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    A component of the human intestinal flora that has been little studied to date is the focus of a new study. Plasmids are small extrachromosomal genetic elements that frequently occur in bacterial cells and can influence microbial lifestyles – yet their diversity in natural habitats is poorly understood. An international team led by Prof. Dr. A. Murat Eren from the Helmholtz Institute for Functional Marine Biodiversity at the University of Oldenburg (HIFMB) reports in the science journal Cell, a mysterious plasmid, is one of the most numerous genetic elements in the human gut that could potentially serve as a powerful biomarker for identifying health hazards such as fecal contamination of water or human disorders such as Inflammatory Bowel Disease. According to the team’s analyses, this plasmid is present in the intestines of more than 90 percent of individuals in industrialized countries.

    Plasmids are extrachromosomal DNA sequences which are common to cells from all domains of life. Eren describes them as “typically small genetic entities that carry additional genes”. They can be exchanged between different bacterial cells and even between different types of bacteria. The replication of plasmids is dependent on their host cells: but they make up for it by providing their hosts with in some cases extremely important fitness determinants. For instance, some plasmids contain genes that encode antibiotic resistance, which help their bacterial hosts to survive antibiotics, contributing one of the most pressing public health concerns around the globe.

    There are also other plasmids which, according to the research to date, do not contain genes encoding obvious beneficial functions for their host. “These so-called ‘cryptic plasmids’ are often referred to as genetic parasites. They remain a mystery in microbial ecology because from an evolutionary perspective they should not exist at all,” explains Eren, a computer scientist and Professor of Ecosystem Data Science at the University of Oldenburg.

    Identifying plasmids has been a difficult undertaking so far. For some time now, scientists have been able to extract genetic material directly from environmental samples and, for example, analyze the microbial community in the human gut in its entirety, without having to cultivate individual bacterial organisms. However, the ability to confidently distinguish what is a plasmid among this conglomeration of genetic material, referred to as the metagenome, poses a considerable challenge.

    To solve this problem, Eren and his colleagues developed a new machine learning approach. As the team reported in an article recently published in the science journal Nature Microbiology, using this approach they identified over 68,000 plasmids in human intestinal flora, and also discovered that a certain cryptic plasmid called pBI143 was particularly abundant in their dataset.

    One of the most numerous genetic entities in the human gut

    In the study published in Cell, the team of researchers took a closer look at this plasmid, which consists of only two genes that rather surprisingly only serves for its own replication and mobilization across bacterial cells with no other clear benefit. To better understand the ecology of pBI143, the team analyzed 60,000 human and 40,000 environmental metagenomes generated from various habitats.

    “We found that pBI143 has a list of very interesting features,” Eren explains. The team discovered that more than 90 percent of people in industrialized countries carry the plasmid and that on average it is one of the most numerous genetic entities in the human gut. “On average it was more than ten times as numerous as a viral genome which was previously thought to be the most abundant genetic extrachromosomal element in the human gut,” says the researcher.

    Further analyses revealed that the plasmid occurred almost exclusively in the human gut but was virtually absent in datasets from other environments such as the oceans, soils, plants and the digestive organs of animals and their feces. The only other samples in which the researchers were able to detect the characteristic gene sequence for these plasmids was in samples from environments that are influenced by humans, such as waste water, hospital surfaces and laboratory rats.

    Due to its sheer numbers, prevalence across humans, and its conservancy across human populations, the team of researchers hypothesized that pBI143 could, for instance, be used as a biomarker in testing for fecal contamination.

    In fact, we were able to show that this plasmid is a more sensitive marker for detecting fecal contamination in drinking water compared to state-of-the-art biomarkers based on specific gene sequences of human intestinal bacteria.”


    Dr. A. Murat Eren, Professor of Ecosystem Data Science at the University of Oldenburg

    Non-invasive method to quantify progress of IBD

    The team also identified another potential application of this prevalent genetic entity in the context of human disorders such as Inflammatory Bowel Disease (IBD), a medical condition that affects 3 million people in Europe alone. They were able to demonstrate that the relative copy number of this cryptic plasmid increased almost four times in the intestines of people suffering from IBD compared as in the intestines of healthy individuals, suggesting that the changes of the copy number of the plasmid can serve as a non-invasive method to quantify the disease progress or severity.

    At the HIFMB, Eren’s team is developing new tools at the intersection of computer science and microbiology to identify and characterize naturally occurring plasmids and other mobile genetic elements in bacteria that live in the ocean. They strive to gain a better understanding of the ecology and evolution of microbes, and strategies that enable to them to respond to their everchanging environments for new biotechnological applications that can ameliorate crises we face.

    Source:

    Journal reference:

    Fogarty, E. C., et al. (2024). A cryptic plasmid is among the most numerous genetic elements in the human gut. Cell. doi.org/10.1016/j.cell.2024.01.039.

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  • Research shows the importance of ‘family’ bonds in care homes

    Research shows the importance of ‘family’ bonds in care homes

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    Care home residents receive much better care when they enjoy ‘family’ bonds with staff – but staff must be empowered to create these bonds, new research has found. 

    The study, led by the University of Leeds and funded by The National Institute for Health and Care Research, found that when care home staffing is stable and consistent and numbers are sufficient, workers have the capacity to develop ‘familial’ relationships with residents and can deliver quality, personalized care. 

    Understanding how to meet the needs and preferences of the thousands of people living in care homes is a societal priority. It is vital that we know how to use the workforce resources in care homes to promote quality and effective working.” 


    Karen Spilsbury, Lead Researcher, Professor of Nursing at the University of Leeds’ School of Healthcare

    Quality of care and quality of life varies significantly for the 441,479 people living in care homes in the UK. Previous research into the reasons for this has not provided robust explanations. 

    The study team speculated that staffing and ways of working were key influences on quality. Working with managers, residents, families, and care home staff from a range of care homes in England, the researchers set out to find out how and why staffing in care homes affects the quality of life and care of the residents. 

    The team analyzed research journal articles, care home and care organization data to look at what it is about staffing that influences quality. They analysed reports and ratings of homes from the Care Quality Commission (CQC) regulator, and networks between staff in homes. 

    According to the results, staffing considerations that might improve quality include not swapping managers too much; having sufficient and consistent staff for family-like relationships in homes and putting residents’ needs first; supporting staff and giving them freedom to act, and key staff leading by example. 

    The research also showed that where more care was provided by registered nurses, there were fewer incidents such as falls with fractures, urinary tract infections and medication errors. 

    However, simply increasing nursing input was unlikely to be a cost-effective way of reducing adverse incidents in care homes. The study found that although there might be savings to the wider healthcare system in reduced treatment costs, any savings would be wiped out by the high additional costs of employing more nurses. 

    The study also found: 

    • Care homes with a manager in-post in the 12 months prior to a CQC inspection were more likely to be rated as good or outstanding 

    • Higher staff-to-bed ratios were associated with a greater chance of a good or outstanding CQC inspection score 

    • Having experienced care staff, that is, staff in post for 5 years, was likely to improve quality, as measured by CQC ratings, and staffing consistency was important for organizing care and work 


    • Larger homes were less likely to be rated positively: but team size (not home size) may be a useful lever for promoting quality, i.e. small groups of linked residents and staff (5–15 residents per staff member based on level of resident dependency) promoted familiarity, communication and a family-like environment for cultivating relationships 

    • Use of agency nurses to cover for staff sickness or unfilled vacancies was not associated with more falls, infections, or pressure ulcers, but was associated with more medication errors 

    The report is the latest publication by NICHE-Leeds, a research partnership between the University’s School of Healthcare and care home providers which develops research projects from ideas generated by care home staff, families and residents. 

    It is the first study in the UK which has worked with a large care organization to provide novel evidence on relationships between nurse staffing and care quality in English care homes over time. 

    Professor Spilsbury, who is also NICHE-Leeds’ Academic Director, added: “Staffing in care homes matters and needs to be valued. It needs to be stable, skilled and competent, to realize the benefits of person-focused organization of care, and enhanced teamworking. 

    “Our study shows that leadership, reward and recognition of staff, and a shared philosophy of care are key to improving quality as experienced by residents.” 

    Source:

    Journal reference:

    Spilsbury, K., et al. (2024) Relationship between staff and quality of care in care homes: StaRQ mixed methods study. Health and Social Care Delivery Research. doi.org/10.3310/GWTT8143.

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  • Natural diversity boosts mental wellbeing, research shows

    Natural diversity boosts mental wellbeing, research shows

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    New research from King’s College London has found that spaces with a diverse range of natural features are associated with stronger improvements in our mental wellbeing compared to spaces with less natural diversity.

    Published in Scientific Reports and funded by the National Institute for Health and Care Research (NIHR) and Wellcome, this citizen science study used the smartphone application Urban Mind to collect real-time reports on mental wellbeing and natural diversity from nearly 2000 participants.

    Researchers found that environments with a larger number of natural features, such as trees, birds, plants and waterways, were associated with greater mental wellbeing than environments with fewer features, and that these benefits can last for up to eight hours.

    Further analysis found that nearly a quarter of the positive impact of nature on mental health could be explained by the diversity of features present. These findings highlight that policies and practices that support richness of nature and species are beneficial both for environment and for public mental health.

    Lead author Ryan Hammoud, Research Assistant at the Institute of Psychiatry, Psychology & Neuroscience (IoPPN), King’s College London, said:

    “To our knowledge, this is the first study examining the mental health impact of everyday encounters with different levels of natural diversity in real-life contexts. Our results highlight that by protecting and promoting natural diversity we can maximise the benefits of nature for mental wellbeing. In practice, this means moving away from heavily curated monocultural pockets and parks of mown grass, which are typically associated with low biodiversity, towards spaces which mirror the biodiversity of natural ecosystems. By showing how natural diversity boosts our mental wellbeing, we provide a compelling basis for how to create greener and healthier urban spaces.”

    The study took place between April 2018 and September 2023, with 1,998 participants completing over 41,000 assessments. Each participant was asked to complete three assessments per day over a period of 14 days, entering information about their environment and answering a series of questions about their mental health. Natural diversity was defined by how many out of four natural features –trees, plants, birds and water – were present within the participant’s surrounding environment.

    Data were collected using the Urban Mind app, developed by King’s College London, landscape architects J&L Gibbons and arts foundation Nomad Projects. The Urban Mind project is funded by a Wellcome Climate Impacts Award to Professor Andrea Mechelli, the National Institute for Health and Care Research (NIHR) Maudsley Biomedical Research Centre and the NIHR Applied Research Collaboration South London.

    Senior author Andrea Mechelli, Professor of Early Intervention in Mental Health at the IoPPN, said:

    “In the context of climate change, we are witnessing a rapid decline in biodiversity in the UK as well as globally. Our results suggest that biodiversity is critical not only for the health of our natural environments but also for the mental wellbeing of the people who live in these environments. It is time to recognize that biodiversity brings co-benefits for planetary and human health and needs to be considered vital infrastructure within our cities”.

    Source:

    Journal reference:

    Hammoud, R., et al. (2024). Smartphone-based ecological momentary assessment reveals an incremental association between natural diversity and mental wellbeing. Scientific Reports. doi.org/10.1038/s41598-024-55940-7.

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  • Teenage pressures and family dynamics influence internalized weight stigma

    Teenage pressures and family dynamics influence internalized weight stigma

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    People who as teenagers felt pressure to lose weight from family or from the media, females, people who are not heterosexual, and people experiencing socioeconomic disadvantage, are most at risk of ‘internalized’ weight stigma, new research led by the University of Bristol has found. The study is published in The Lancet Regional Health Europe today [15 April].

    ‘Internalised’ weight stigma, is when people apply negative obesity-related stereotypes to themselves, such as thinking they are less attractive, less competent, or less valuable as a person because of their weight. This is the first time a study has used a large UK sample to examine who is most at risk.

    In England, around one in four people are living with obesity, but it is highly stigmatized. Negative obesity-related stereotypes and weight-related discrimination are widespread in society. Experience of weight stigma is a major public health issue: people affected by weight stigma are more likely to have poor mental health, eating disorders and may delay seeking medical treatment. However, little is known about which population groups are at higher risk of internalized weight stigma, because previous research has used small, nonrepresentative samples. 

    Using data from Bristol’s Children of the 90s (also known as the Avon Longitudinal Study of Parents and Children), this new study examined differences in internalized weight stigma in over 4,000 people aged 31 years by sex, ethnicity, socioeconomic factors, sexual orientation, and family and wider social influences in childhood and adolescence.

    The research found that feeling pressure from family to lose weight, weight-related teasing by family members, and feeling pressure from the media to lose weight as a teenager were all linked to higher levels of internalized weight stigma at age 31, and this was not explained by differences in body mass index (BMI). Being bullied in adolescence (at age 17 years) and adulthood (at age 23 years) were also independently linked to internalized weight stigma at age 31.

    The study also found there are clear differences in internalized weight stigma between other groups of the population, which were also not explained by differences in BMI. Females and people who did not identify as heterosexual are at greater risk of internalized weight stigma. People who had spent more of their 20’s as NEETs (not in education, employment or training), or whose mothers had fewer educational qualifications, are also more at risk of internalized weight stigma.

    The family environment in adolescence, bullying, and pressure to lose weight from the media may have long-lasting impacts on how people value themselves based on their weight as adults.


    We have an opportunity to reduce weight stigma and its consequences by changing how we discuss weight in the media, in public spaces and in families, and how we respond to bullying in schools, workplaces, and other settings.


    This is crucial considering how common pressure to lose weight and weight-related bullying, stigma and discrimination are in many cultures around the world.”


    Dr Amanda Hughes, Research Fellow in the Bristol Medical School: Population Health Sciences (PHS) and corresponding author

    The researchers now plan to explore in detail the psychological processes by which these social factors may influence internalized weight stigma.

    The research was funded by the Economic and Social Research Council (ESRC), Medical Research Council (MRC), National Institute for Health and Care Research (NIHR), and Wellcome Trust. 

    Source:

    Journal reference:

    Hughes, A. M., et al. (2024) Demographic, socioeconomic and life-course risk factors for internalized weight stigma in adulthood: evidence from an English birth cohort study. The Lancet Regional Health – Europe. doi.org/10.1016/j.lanepe.2024.100895.

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  • Innovative testing protocol reduces the lengthy wait for IBD diagnosis

    Innovative testing protocol reduces the lengthy wait for IBD diagnosis

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    Patients with suspected inflammatory bowel disease (IBD) could benefit from better testing protocols that would reduce the need and lengthy wait for potentially unnecessary colonoscopies, a new study has found.

    In a paper published in Frontline Gastroenterology, researchers from the Birmingham NIHR Biomedical Research Centre (BRC) at the University of Birmingham tested a new protocol to improve IBD diagnosis combining clinical history with multiple home stool tests.

    In the two-year study involving 767 participants, patients were triaged and had repeated fecal calprotectin (FCP) tests and the research team found that the use of serial FCP tests were able to strongly predict possible IBD as well as Crohn’s Disease and Ulcerative Colitis.

    The team observed that a second FCP test was a strong indicator of a potential need for further investigation including colonoscopy; although the researchers observed that only 20% of patients had two samples submitted prior to referral to secondary care.

    Dr Peter Rimmer from the Birmingham NIHR Biomedical Research Centre at the University of Birmingham and corresponding author of the study said:

    “Patients who experience symptoms associated with inflammatory bowel diseases often have a long wait until getting a diagnosis, and current testing is under immense strain.

    “Using a comprehensive 13-point symptom checker and multiple FCP tests, we have been able to identify much more accurately patients who had IBD and other diseases. The rollout of this protocol could reduce the time taken to get a diagnosis and start treatment for IBDs as much more of the screening and testing can be done through primary care. The sensitivity of multiple FCP tests can be used to flag those patients who urgently need referral into secondary care.”

    In its simplest form, this study may help improve referral triage for IBD patients. But as we plan new care pathways, it could open up new exciting possibilities: with the growing availability of home FCP testing, these tests’ results combined with simple symptom questionnaires could feed into algorithms that allow patients to self-refer to secondary care services, reducing strain on primary care. This is something we’re going to explore in a large follow-up study we’re currently initiating.”


    Dr Rachel Cooney, Consultant Gastroenterologist at University Hospitals Birmingham NHS Foundation Trust, researcher at the NIHR Birmingham BRC and co-author of the study

    Source:

    Journal reference:

    Rimmer, P., et al. (2024). Optimising triage of urgent referrals for suspected IBD: results from the Birmingham IBD inception study. Frontline Gastroenterology. doi.org/10.1136/flgastro-2023-102523.

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  • Transforming breast cancer care globally

    Transforming breast cancer care globally

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    The new Lancet Commission on Breast Cancer today released findings and recommendations on improving breast cancer care globally. Over the past two years, the international multidisciplinary group of experts, including Dr Fatima Cardoso, president of the Advanced Breast Cancer (ABC) Global Alliance, has worked to transform the breast cancer care landscape. Supported and funded in part by the ABC Global Alliance, the commission has led debate around key issues, supported pilot studies, culminating it its landmark report advocating for six crucial areas for change.

    One such areas in metastatic or advanced breast cancer. The report notes that 20 to 30% of patients with early breast cancer suffer recurrences that are not usually documented by most national oncology registries. As a result, the number of patients living with metastatic breast cancer, already a poorly understood disease, is unknown. The authors argue that at least 70% of oncology registries worldwide must better document the stage of the disease upon diagnosis, as well as relapses, to accurately reflect the prevalence and impact of the disease. This would be supported by the improvement of early diagnosis programs, with the commission aiming for 60% of invasive cancer cases diagnosed in stage I or II.

    Patients living with metastatic/advanced breast cancer (MBC/ABC) have long suffered from stigma, misconceptions, and abandonment not just from society in general but also from healthcare providers and patient advocacy groups. There is an urgent need for change in the way metastatic breast cancer is understood and managed. With this change, it will be possible to treat most, alleviate suffering for all, and forget no-one living with MBC/ABC. This is one of the strongest messages from the new Lancet Commission on Breast Cancer, which is totally aligned with the long-lasting fight from the ABC Global Alliance.”


    Dr. Fatima Cardoso, the ABC Global Alliance President and Director of the Breast Unit at the Champalimaud Clinical Center, Lisbon, Portugal

    The report also stresses the importance of improving communication with patients, stating all healthcare professionals in the world should receive training in communication skills to allow them to better involve patients in all stages of cancer care.

    The report further states there are many costs – physical, psychological, social and financial – that are poorly recognized or not captured by current global health indicators, a gap that the Commission argues must be addressed urgently by developing new tools and metrics.

    The new Lancet Commission on Breast Cancer started to work in 2021 with the commitment to raise the standard of breast cancer care and to close the equity gap that exists between and within countries. The recommendations to face the urgent challenges for breast cancer patients will be released this Tuesday, April 16, at 2 pm, in an online session.

    Registration can be done via the link: https://www.eventbrite.co.uk/e/lancet-breast-cancer-commission-report-launch-tickets-862423932197).

    The Commission’s work was funded and supported by the National Institute for Health and Care Research (NIHR), Breast Cancer Now, the ABC Global Alliance and the University of Cambridge.

    Source:

    Journal reference:

    Coles, C. E., et al. (2024) The Lancet Breast Cancer Commission. The Lancet. doi.org/10.1016/S0140-6736(24)00747-5.

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  • California health workers may face rude awakening with $25 minimum wage law

    California health workers may face rude awakening with $25 minimum wage law

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    Nearly a half-million health workers who stand to benefit from California’s nation-leading $25 minimum wage law could be in for a rude awakening if hospitals and other health care providers follow through on potential cuts to hours and benefits.

    A medical industry challenge to a new minimum wage ordinance in one Southern California city suggests layoffs and reductions in hours and benefits, including cuts to premium pay and vacation time, could be one result of a state law set to begin phasing in in June. However, some experts are skeptical of that possibility.

    The California Hospital Association brought a partly successful legal challenge to Inglewood’s $25 minimum wage ordinance, which barred employers from taking those sorts of steps to offset their higher costs.

    “Layoffs, reductions in premium pay rates, reductions in non-wage benefits, reductions in hours, and increased charges are consequences of an employer having less money to spend—which will necessarily be the case given the significant increase in spending on wages due to the minimum wage,” the association said in its lawsuit. Additional examples include reducing health coverage and charging for parking or work-related equipment.

    Inglewood voters approved the ordinance in November 2022, nearly a year before California legislators enacted a $25 minimum wage for health workers. Those statewide higher wages are to be phased in starting in June under California’s first-in-the-nation law, but Gov. Gavin Newsom has since said they are too expensive as the state faces a deficit estimated between $38 billion and $73 billion. It’s unclear if lawmakers will agree to a delay or take other steps to reduce the cost.

    U.S. District Judge Dale S. Fischer agreed with the hospital industry in a March 11 tentative ruling when he shot down the portion of Inglewood’s ordinance banning layoffs and clawbacks by employers, while allowing the rest of the ordinance to remain in effect. He gave the sides time to object to his preliminary decision, though none did.

    The California Hospital Association represents more than 400 hospitals and was a key backer of the state’s carefully crafted compromise law, which notably contains none of the employee safeguards included in the Inglewood ordinance.

    Spokesperson Jan Emerson-Shea said the association doesn’t know how providers will react once the state law takes effect. “We don’t have any insights,” she said.

    “The challenge for any health care organization is figuring out how to pay for the higher wages,” said Joanne Spetz, director of the Philip R. Lee Institute for Health Policy Studies at the University of California-San Francisco. “Since labor costs are the largest part of any health care organization’s costs, it’s hard to figure out how to reduce spending without looking at labor costs.”

    Providers can try to increase revenues by bargaining for higher reimbursements from commercial insurers, she said. Public hospitals, nursing homes, and community clinics get most of their money through Medi-Cal, the state’s Medicaid program.

    Providers could reduce the services they offer, pare back charity care, and cut or delay capital investments, Spetz said. In the long term, she expects some combination of spending cuts and revenue increases.

    Both the state law and local ordinance cover far more than doctors and nurses, with a definition of health worker that includes janitors, housekeepers, groundskeepers, security guards, food service workers, laundry workers, and clerical staff.

    The most recent estimate by the Health Care Program at the University of California-Berkeley Labor Center is that as many as 426,000 health workers would make an average of $6,400 extra in the law’s first year, a 19% average pay bump mainly benefiting lower-income workers of color and women. State finance officials project that well over 500,000 workers will benefit.

    Researchers didn’t include layoffs and other potential staffing and benefit reductions when they projected the state law’s costs and benefits, said Laurel Lucia, the program’s director. But she pointed to initial projections by hospitals, doctors, and business and taxpayer groups that the wage hike would cost $8 billion annually, thereby imperiling services and resulting in higher premiums and higher costs for state and local governments.

    “It seems like a contradiction to say this law’s going to cost billions of dollars while at the same time saying it’s going to reduce workers’ total compensation,” said Lucia, who projects a far lower price tag.

    She added that state finance officials had anticipated that Medi-Cal reimbursements would reflect the increased labor costs, while Medicare would eventually at least partially compensate for the higher labor costs.

    Michael Reich, chair of the Center on Wage and Employment Dynamics at UC Berkeley’s Institute for Research on Labor and Employment, and affiliated economist Justin Wiltshire recently argued that California’s new $20 minimum wage law for fast-food workers won’t result in mass layoffs and price increases, as some have predicted.

    Health care is much different than fast food, Reich acknowledged, but he argued for much the same positive result.

    “A higher minimum wage will make it easier and cheaper for hospitals to recruit and retain these workers. The cost savings, and the productivity benefits of more experienced workers, could offset much of the labor cost increase,” Reich said.

    The hospital association filed its lawsuit against Inglewood’s ordinance in July, while it was still opposing early versions of the statewide minimum wage legislation. Among many other provisions, the statewide law put on hold an initiative to cap hospital executives’ salaries in Los Angeles.

    The hospital association’s legal challenge referenced in part layoffs and reduced working hours imposed by Centinela Hospital Medical Center after Inglewood’s ordinance took effect.

    But Centinela said the reduction was entirely unrelated to the ordinance and that all staff were offered alternate positions, which many accepted.

    “Centinela Hospital also has since added many more jobs in new clinical positions above minimum wage scale,” the hospital said in a statement.

    Service Employees International Union-United Healthcare Workers West, the prime backer of both the local ordinance and the statewide law, sued the hospital in April 2023 alleging that it cut workers’ hours to offset the higher minimum wage. The case is still pending.

    The union did not respond to repeated requests for comment.

    In a court filing, however, the union and city of Inglewood said similar employer restrictions in previous minimum wage laws have survived.

    The ordinance “merely sets the backdrop for collective bargaining negotiations,” and does not bar employers from locking out employees or hiring replacement workers during a strike. Employers can still lay off workers or reduce their hours, they said, so long as they don’t do so to fund the higher minimum wage.

    But Fischer agreed with the hospital association that layoffs and reductions in employees’ total compensation packages are “obvious responses by an employer to rising compensation costs.”

    Restricting employers’ options would violate federal labor relations rules, he said.

    “The minimum wage an employer has to pay its employees will invariably affect the total amount of compensation it is able or willing to pay,” he wrote “This will then invariably affect the number of employees it can retain and the number of hours those employees will be scheduled to work.”

    This article was produced by KFF Health News, which publishes California Healthline, an editorially independent service of the California Health Care Foundation. 




    Kaiser Health NewsThis article was reprinted from khn.org, a national newsroom that produces in-depth journalism about health issues and is one of the core operating programs at KFF – the independent source for health policy research, polling, and journalism.

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  • Google Gemini and Bard pass the ophthalmology board examination

    Google Gemini and Bard pass the ophthalmology board examination

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    In a recent study published in the journal Eye, researchers from Canada evaluated the performance of two artificial intelligence (AI) chatbots, Google Gemini and Bard, in the ophthalmology board examination.

    They found that both the tools achieved acceptable accuracy in the answers and performed well in the field of ophthalmology, with some variation across countries.

    Study: Google Gemini and Bard artificial intelligence chatbot performance in ophthalmology knowledge assessment. Image Credit: Deemerwha studio/Shutterstock.comStudy: Google Gemini and Bard artificial intelligence chatbot performance in ophthalmology knowledge assessment. Image Credit: Deemerwha studio/Shutterstock.com

    Background

    AI chatbots such as ChatGPT (short for chat-generative pre-trained transformer), Bard, and Gemini are increasingly used in medical settings. Their performance continues to evolve across exams and disciplines.

    While ChatGPT-3.5’s accuracy was up to 64% in steps one and two of the AMBOSS and NBME (short for National Board Medical Examination) exams, newer versions like ChatGPT-4 showed improved performance.

    Google’s Bard and Gemini offer responses based on diverse cultural and linguistic training, potentially tailoring information to specific countries. However, the responses vary across geographies, calling for further research to ensure consistency, particularly in medical applications where accuracy is crucial for patient safety.

    In the present study, researchers aimed to evaluate the performance of Google Gemini and Bard on a set of practice questions designed for the ophthalmology board certification exam.

    About the study

    The performance of Google Gemini and Bard was assessed using 150 text-based multiple-choice questions obtained from “EyeQuiz,” an educational platform for medical professionals specializing in ophthalmology.

    The portal provides practice questions for various exams, including the Ophthalmic Knowledge Assessment Program (OKAP), national board exams such as the American Board of Ophthalmology (ABO) exam, as well as certain postgraduate exams.

    The questions were categorized manually, and data were collected using the Bard and Gemini versions available as of 30th November and 28th December 2023, respectively. The accuracy, provision of explanations, response time, and question length were assessed for both tools.

    Secondary analyses included evaluating the performance in countries other than the United States (US), including Vietnam, Brazil, and the Netherlands, using virtual private networks (VPNs).

    Statistical tests, including the chi-square and Mann-Whitney U tests, were conducted to compare performance across countries and chatbot models. Multivariable logistic regression was used to explore factors influencing correct responses.

    Results and discussion

    Bard and Gemini responded promptly and consistently to all 150 questions without experiencing high demand. In the primary analysis using the US versions, Bard took 7.1 ± 2.7 seconds to respond, while Gemini responded in 7.1 ± 2.8 seconds, with a longer average response length.

    In the primary analysis using the US form of the chatbots, both Bard and Gemini achieved an accuracy of 71%, correctly answering 106 out of 150 questions. Bard provided explanations for 86% of its responses, while Gemini provided explanations for all responses.

    Bard was found to perform best in orbital & plastic surgery, while Gemini showed superior performance in general ophthalmology, orbital & plastic surgery, glaucoma, and uveitis. However, both the tools struggled in the cataract & lenses and refractive surgery categories.

    In the secondary analysis with Bard from Vietnam, the chatbot answered 67% of questions correctly, similar to the US version. However, using Bard from Vietnam led to different answer choices in 21% of questions compared to the US version.

    With Gemini from Vietnam, 74% of questions were answered correctly, similar to the US version, but there were differences in answer choices for 15% of questions compared to the US version. In both cases, some questions answered incorrectly by the US versions were answered correctly by the Vietnam versions, and vice versa.

    The Vietnam versions of Bard and Gemini explained 86% and 100% of their responses, respectively. Bard performed best in retina & vitreous and orbital & plastic surgery (80% accuracy), while Gemini performed better in cornea & external disease, general ophthalmology, and glaucoma (87% accuracy each).

    Bard struggled most in cataracts & lenses (40% accuracy), while Gemini faced challenges in pediatric ophthalmology & strabismus (60% accuracy). Gemini’s performance in Brazil and the Netherlands was relatively inferior to the US and Vietnam versions.

    Despite the promising findings, the study’s limitations include a small question sample size, reliance on an openly accessible question bank, unexplored effects of user prompts, internet speed, website traffic on response times, and occasional incorrect explanations provided by the chatbots.

    Future studies could explore the chatbots’ ability to interpret ophthalmic images, which remains relatively unexplored. Further research is warranted to address the limitations and explore additional applications in the field.

    Conclusion

    In conclusion, although both the US and Vietnam iterations of Bard and Gemini demonstrated satisfactory performance on ophthalmology practice questions, the study highlights potential response variability linked to user location.

    Future evaluations to track the enhancement of AI chatbots and comparisons between ophthalmology residents and AI chatbots could offer valuable insights into their efficacy and reliability.

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  • Persistent measles importations challenge U.S. elimination efforts

    Persistent measles importations challenge U.S. elimination efforts

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    A recent Centers for Disease Control and Protection (CDC) report published in the Morbidity and Mortality Weekly Report (MMWR) assessed the status of measles elimination in the United States (U.S.) and reported on the recent increase in cases by analyzing surveillance data from January 2020 to March 2024.

    Study: Measles — United States, January 1, 2020–March 28, 2024. Image Credit: sulit.photos/Shutterstock.comStudy: Measles — United States, January 1, 2020–March 28, 2024. Image Credit: sulit.photos/Shutterstock.com

    Background 

    Measles, a highly contagious viral illness, was considered eliminated in the U.S. in 2000 due to high coverage with the measles, mumps, and rubella (MMR) vaccine.

    Despite this, the elimination status faced challenges in 2019 following significant outbreaks in under-vaccinated communities in New York, contributing to a notable rise in cases.

    Further research is needed to address gaps in vaccine coverage and enhance surveillance systems to prevent future outbreaks and sustain measles elimination.

    About the study 

    Confirmed measles cases are reported to the Centers for Disease Control and Prevention (CDC) by state health departments through the National Notifiable Disease Surveillance System and directly via email or telephone to the National Center for Immunization and Respiratory Diseases.

    The Council of State and Territorial Epidemiologists classifies measles cases. Cases are deemed import-associated if they originate internationally, are epidemiologically linked to an imported case, or show viral genetic evidence of an imported measles genotype.

    Conversely, cases without an epidemiologic or virologic connection to an imported source are categorized as having an unknown origin.

    For analytical purposes, unique sequences are identified as those varying by at least one nucleotide in the N-450 sequence, following the World Health Organization’s (WHO)’s recommendations for describing sequence variants.

    Patients who were unvaccinated yet eligible for vaccination were identified based on the criteria set by the Advisory Committee on Immunization Practices.

    A surveillance system is considered effective if at least 80% of cases meet three criteria: classification as import-associated, comprehensive reporting on key surveillance indicators, and confirmation through laboratory testing.

    Additionally, measles cases are categorized into chains of transmission based on known epidemiologic links. These include isolated cases, two-case chains involving two linked cases, and outbreaks consisting of three or more linked cases.

    The analysis of two-case chains and outbreaks also involves assessing the potential for missed cases by examining the time intervals between the onset of measles rashes, with intervals exceeding one maximum incubation period suggesting possible unreported cases. 

    Study results 

    From January 1, 2020, to March 28, 2024, the CDC was notified of 338 confirmed measles cases across 30 jurisdictions. A noticeable portion of these cases, particularly 12 of 13 reported before the COVID-19 mitigation measures began in March 2020, marked the early stages of this period.

    In the following years, 2021 and 2022 witnessed 170 reported cases, 78% of which were linked to distinct outbreaks. Notably, 47 of the 49 cases in 2021 were among Afghan evacuees at U.S. military bases during Operation Allies Welcome, and 86 of the 121 cases in 2022 stemmed from an outbreak in Central Ohio.

    By 2023, 48% of the 58 cases reported were associated with four outbreaks. As of late March 2024, 97 cases had been reported for the year, signifying a significant increase over previous first-quarter averages.

    The median age of the affected individuals was three years, ranging from newborns to 64 years old, with 58% of the cases occurring in the 16 months to 19 years age group.

    A significant majority, 91% of patients, were either unvaccinated or had an unknown vaccination status, and of these, 84% were eligible for vaccination.

    Hospitalization data showed that 46% of the patients were hospitalized, primarily children under five years old, with 92% being unvaccinated or of unknown vaccination status. There were no reported deaths due to measles.

    Regarding the origin of the cases, 96% were linked to importation. A detailed breakdown reveals that the majority of the 326 import-related cases involved U.S. residents eligible yet unvaccinated or of unknown vaccination status.

    The most frequent origins of these imported cases were from the Eastern Mediterranean and African WHO regions. However, the first quarter of 2024 saw increased European and Southeast Asian cases.

    Surveillance quality was notably high, with nearly all cases (98%) including comprehensive data on key indicators. Timeliness of reporting showed that 58% of cases were reported to health departments on or before the day of rash onset.

    Laboratory confirmations were achieved in 93% of cases, with a large majority verified through real-time reverse transcription-polymerase chain reaction (rRT-PCR) testing and successful genotyping of most specimens, identifying predominant measles genotypes B3 and D8.

    Transmission patterns were categorized into 92 chains, with the majority being isolated cases. However, 20 chains qualified as outbreaks involving three or more cases, and the typical outbreak lasted 20 days, highlighting the rapid transmission potential of measles.

    Notably, none of the two-case chains or larger outbreaks experienced a gap exceeding the maximum incubation period.

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