Tag: Tobacco

  • Cost becomes a significant factor in smoking cessation efforts

    Cost becomes a significant factor in smoking cessation efforts

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    Health concerns are still the primary motive for more than half of those who say they want to stop smoking in England, but cost is now a key factor for more than 1 in 4, finds an analysis of national survey responses, published in the open access journal BMJ Public Health.

    Given this shift in thinking, making much more of the potential savings to be had might encourage more people to stub out for good, suggest the researchers.

    Health concerns are generally the primary motive for people trying to stop smoking, with social and financial concerns, plus advice from a health professional, also commonly cited reasons, explain the researchers.

    But since 2020, England has undergone a period of substantial societal instability, prompted primarily by the COVID-19 pandemic, which might have triggered changes in the reasons smokers give for wanting to ditch tobacco, they suggest.

    To find out, the researchers looked at time trends in motives for trying to stop smoking between March 2018 and May 2023, exploring differences by age, sex, socioeconomic status, presence of children in the household and vaping status.

    They drew on responses to the ongoing Smoking Toolkit Study, a monthly survey of a representative sample of around 1700 adults in England.

    The responses were limited to those who were either current smokers or who had stopped smoking in the past year and had made at least one serious attempt to quit during that time.

    Respondents were asked to name the reason(s) behind their most recent quit attempt from among: advice from a health professional; TV advert for a nicotine replacement product; government TV/radio/press advert; a new stop smoking treatment; cost; smoking restrictions; knowing someone else who was quitting; health warning on a cigarette packet; contact from a local NHS stop smoking service; current or future health problems; attending a local stop smoking activity or event; comments by family, friends, children; significant birthday; pregnancy; simple decision to quit; COVID-19 pandemic.

    Out of the 101,919 survey respondents between 2018 and 2023, 17,812 reported smoking in the past year. Of these,17,031 (96%) provided data on quit attempts over the past 12 months, 5777 (34%) of whom reported having made at least one serious attempt to do so.

    Health concerns were the most frequently cited motives, reported by more than half the sample (52%) across the entire period—especially concerns about future health, reported by more than 1 in 3 (35.5%) compared with 1 in 5 (19%) who were motivated by current health problems.

    Cost was the next most frequently cited motive, reported by nearly 1 in 4 (23%), followed by social factors, reported by around 1 in 5 (19%) and advice from a health professional (12%). 

    Around 4% said they were motivated by health warnings on a cigarette packet, while smoking restrictions prompted 3.5% to try and stop; a simple decision to quit was cited by just over 3%. The other reasons attracted only around 1% each.

    Up to the start of 2020, 1 in 2 quit attempts was motivated by health concerns; 1 in 5 by current health problems (20%), and 1 in 3 by concerns about future health (34%). One in 5 was motivated by social factors (20%) and cost (20%), and 1 in 6 by health professional advice (16.5%).

    While there was little overall change in the proportion of quit attempts motivated by health concerns across the entire study period, the proportion of quit attempts motivated by cost increased significantly, rising from just over 19% in March 2018 to just under 25.5% in May 2023.

    But the proportion of quit attempts motivated by health professional advice fell significantly over the entire study period, dropping from just over 14% in March 2018 to 8.5% in May 2023.

    The COVID-19 pandemic, which began to affect England in March 2020, is likely to have influenced the proportion of respondents reporting health concerns, social factors, and cost as motives for trying to stop smoking, suggest the researchers. 

    The proportion of quit attempts motivated by future health concerns increased during 2020 and 2021.”It is likely the pandemic made health concerns (an already prevalent motive) even more salient, particularly during its first year when the virus was spreading rapidly and vaccinations were not yet available,” they write. 

    Once the immediate threat of the virus had subsided thanks to the vaccination programme, the proportion of health-related attempts to quit returned to pre-pandemic levels.

    The pandemic probably influenced other motives, suggest the researchers, because it prompted loss of income and jobs for many people.

    “These economic pressures probably contributed to the rise in cost-motivated attempts to quit around this time. But while the pandemic’s acute risks to health—and, as a result, attempts to quit motivated by concern for health or social factors—waned over time, its economic impacts have been compounded by a cost-of- living crisis,” they explain.

    The pandemic’s impact on access to, and availability of, healthcare services may also have contributed to the decline in the proportion of respondents citing healthcare professional advice as a motivating factor, they add.

    The researchers acknowledge various caveats to their findings, including that all the study data were self-reported and relied on personal recall, and may not apply to other countries with different attitudes to smoking, tobacco control policies, and provision of smoking cessation services.

    But they conclude: “These findings have implications for smoking cessation interventions and clinical practice. ..They indicate that cost is an increasingly important factor motivating people to try to stop smoking. 

    “Communicating the potential savings people can make by stopping smoking (even if they switch to alternative nicotine products) could therefore be an effective means for motivating attempts to quit.” 

    Source:

    Journal reference:

    Jackson, S. E., et al. (2024). Trends in motives for trying to stop smoking: a population study in England, 2018–2023. BMJ Public Health. doi.org/10.1136/bmjph-2023-000420.

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  • UC San Diego researchers discover genetic connections to alcohol consumption

    UC San Diego researchers discover genetic connections to alcohol consumption

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    A research group centered at the University of California San Diego School of Medicine has drilled deep into a dataset of over 3 million individuals compiled by the direct-to-consumer genetics company 23andMe, Inc., and found intriguing connections between genetic factors influencing alcohol consumption and their relationship with other disorders.

    The study was recently published in the Lancet eBioMedicine.

    Sandra Sanchez-Roige, Ph.D., corresponding author and associate professor at UC San Diego School of Medicine Department of Psychiatry, explained that the study used genetic data to broadly classify individuals as being European, Latin American and African American. Such classifications “are needed to avoid a statistical genetics pitfall called population stratification,” noted co-author Abraham A. Palmer, Ph.D., professor and vice chair for basic research in the psychiatry department.

    The researchers analyzed genetic data from the 3 million 23andMe research participants, focusing on three specific little snippets of DNA known as single-nucleotide polymorphisms, or SNPs. Sanchez-Roige explained that variants, or alleles, of these particular SNPs are “protective” against a variety of alcohol behaviors, from excessive alcohol drinking to alcohol use disorder.

    One of the alcohol-protective variants they considered is very rare: the most prevalent among the three alleles found in the study showed up in 232 individuals of the 2,619,939 European cohort, 29 of the 446,646 Latin American cohort and in 7 of the 146,776 African American cohort; others are much more common. These variants affect how the body metabolizes ethanol -; the intoxicating chemical in alcoholic beverages.

    The people who have the minor allele variant of the SNP convert ethanol to acetaldehyde very rapidly. And that causes a lot of negative effects.”


    Sandra Sanchez-Roige, Ph.D., corresponding author and associate professor at UC San Diego School of Medicine Department of Psychiatry

    She went on to say that the resulting nausea eclipses any pleasurable effects of alcohol -; think of a bad hangover that sets in almost immediately.

    “These variants are primarily associated with how much someone may consume alcohol,” she said. “And they also tend to prevent alcohol use disorder, because these variants are primarily associated with the quantity of alcohol someone may drink.”

    Sanchez-Roige explained that the SNP variants’ influence on alcohol consumption are well researched, but her group took a “hypothesis-free” approach to the 23andMe dataset, which contains survey data on thousands of traits and behaviors. The researchers wanted to find out if the three SNP variants might have any other effects beyond alcohol consumption.

    Sanchez-Roige and Palmer noted that their group has developed a 10-year partnership with 23andMe that has focused on numerous traits, especially those with relevance for addiction. This work is the basis of an academic collaboration through the 23andMe Research Program. 

    They data-mined the analyses of DNA from saliva samples submitted by consenting 23andMe research participants, as well as the responses to the surveys of health and behavior available from the 23andMe database, and found a constellation of associations, not necessarily connected with alcohol. Individuals with the alcohol-protecting alleles had generally better health, including less chronic fatigue and needing less daily assistance with daily tasks.

    But the paper notes individuals with the alcohol-protective alleles also had worse health outcomes in certain areas: more lifetime tobacco use, more emotional eating, more Graves’ disease and hyperthyroidism. Individuals with the alcohol-protective alleles also reported totally unexpected differences, such as more malaria, more myopia and several cancers, particularly more skin cancer and lung cancer, and more migraine with aura. 

    Sanchez-Roige acknowledged that there is a chicken-and-egg aspect to their findings. For example: Cardiovascular disease is just one of a number of maladies known to be associated with alcohol consumption. “So is alcohol consumption leading to these conditions?” she asks. Palmer finishes the thought: “Or do these genetic differences influence traits like malaria and skin cancer in a manner that is independent of alcohol consumption?”

    Sanchez-Roige said that such broad, hypothesis-free studies are only possible if researchers have access to very large sets of data. Many datasets, including the one used in the study, rely heavily on individuals with European ancestry.

    “It is important to include individuals from different ancestral backgrounds in genetic studies because it provides a more complete understanding of the genetic basis of alcohol behaviors and other conditions, all of which contributes to a more inclusive and accurate understanding of human health,” she said. “The study of only one group of genetically similar individuals (for example, individuals of shared European ancestry) could worsen health disparities by aiding discoveries that will disproportionately benefit only that population.”

    She said their study opens numerous doors for future research, chasing down possible connections between the alcohol-protective alleles and conditions that have no apparent connection with alcohol consumption.

    “Understanding the underlying mechanisms of these effects could have implications for treatments and preventative medicine,” Sanchez-Roige noted. 

    Co-authors on the paper from the University of California San Diego School of Medicine Department of Psychiatry are Mariela V. Jennings, Natasia S. Courchesne-Krak, Renata B. Cupertino and Sevim B. Bianchi. Sandra Sanchez-Roige is also associated with the Department of Medicine, Division of Genetic Medicine, Vanderbilt University.

    Other co-authors are: José Jaime Martínez-Magaña, Department of Psychiatry, Division of Human Genetics, Yale University School of Medicine; Laura Vilar-Ribó, Psychiatric Genetics Unit, Group of Psychiatry, Mental Health and Addiction, Vall d’Hebron Research Institute, Universitat Autònoma de Barcelona, Barcelona, Spain; Alexander S. Hatoum, Department of Psychology & Brain Sciences, Washington University in St. Louis; Elizabeth G. Atkinson, Department of Molecular and Human Genetics, Baylor College of Medicine; Paola Giusti-Rodriguez, Department of Psychiatry, University of Florida College of Medicine; Janitza L. Montalvo-Ortiz, Department of Psychiatry, Division of Human Genetics, Yale University School of Medicine, National Center of Posttraumatic Stress Disorder, VA CT Healthcare Center; Joel Gelernter, VA CT Healthcare Center, Department of Psychiatry, West Haven CT; and Departments of Psychiatry, Genetics & Neuroscience, Yale Univ. School of Medicine; María Soler Artigas, Psychiatric Genetics Unit, Group of Psychiatry, Mental Health and Addiction, Vall d’Hebron Research Institute, Universitat Autònoma de Barcelona, Barcelona, Spain; Department of Mental Health, Hospital Universitari Vall d’Hebron, Barcelona; Biomedical Network Research Centre on Mental Health (CIBERSAM), Madrid; and Department of Genetics, Microbiology, and Statistics, Faculty of Biology, Universitat de Barcelona; Howard J. Edenberg, Department of Biochemistry and Molecular Biology, Indiana University School of Medicine; and the 23andMe Inc. Research Team, including Sarah L. Elson and Pierre Fontanillas.

    The study was funded, in part, by Tobacco-Related Disease Research Program grants T32IR5226 and 28IR-0070, National Institute of Health (NIH) National Institute of Drug Abuse (NIDA) DP1DA054394, and NIH National Institute of Mental Health (NIMH) R25MH081482. 

    Source:

    Journal reference:

    Jennings, M. V., et al. (2024) A phenome-wide association and Mendelian randomisation study of alcohol use variants in a diverse cohort comprising over 3 million individuals. Lancet eBioMedicine. doi.org/10.1016/j.ebiom.2024.105086.

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  • Florida limits abortion — for now

    Florida limits abortion — for now

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    The host

    Julie Rovner KFF Health News @jrovner

    Julie Rovner is chief Washington correspondent and host of KFF Health News’ weekly health policy news podcast, “What the Health?” A noted expert on health policy issues, Julie is the author of the critically praised reference book “Health Care Politics and Policy A to Z,” now in its third edition.

    Florida this week became a major focus for advocates on both main sides of the abortion debate. The Florida Supreme Court simultaneously ruled that the state’s 15-week ban, passed in 2022, can take effect immediately before a more sweeping, six-week ban replaces it in May and that voters can decide in November whether to create a state right to abortion.

    Meanwhile, President Joe Biden, gearing up for the general election campaign, is highlighting his administration’s health accomplishments, including drug price negotiations for Medicare.

    This week’s panelists are Julie Rovner of KFF Health News, Joanne Kenen of the Johns Hopkins University schools of nursing and public health, Tami Luhby of CNN, and Lauren Weber of The Washington Post.

    Panelists

    Among the takeaways from this week’s episode:

    • The Florida Supreme Court’s decisions this week will affect abortion access not only in the state, but also throughout the region. Florida’s six-week ban, which takes effect on May 1, would leave North Carolina and Virginia as the only remaining Southern states offering the procedure beyond that point in pregnancy — and, in North Carolina, abortion is banned at 12 weeks after a woman’s last menstrual period.
    • Since the U.S. Supreme Court overturned the constitutional right to an abortion in 2022, six states have voted on their own constitutional amendments related to abortion access. In every case, the side favoring abortion rights has won. But Florida’s measure this fall will appear on the ballot with the presidential race. Could the two contests, waged side by side, boost turnout and influence the results?
    • Former President Donald Trump made many attempts during his term to undermine the Affordable Care Act, and this week the Biden administration reversed another one of those lingering attempts. Under a new regulation, the use of short-term insurance plans will be limited to four months — down from 36 months under Trump. The plans, which Biden officials call “junk plans” due to their limited benefits, will also be required to provide clearer explanations of coverage to consumers.
    • In other Biden administration news, March has come and gone without the release of an anticipated ban on menthol flavoring in tobacco, and anti-tobacco groups are suing to force administration officials to finish the job. Menthol cigarettes are particularly popular in the Black community, and — like Trump’s decision as president to punt a ban on vaping to avoid alienating voters in 2020 — the Biden administration may be loath to raise the issue this year. Activists say, however, that it may be at the expense of Black lives.
    • “This Week in Medical Misinformation” looks at an article from PolitiFact about the health misinformation that persists even with the pandemic mostly in the rearview mirror.

    Also this week, Rovner interviews health care analyst Jeff Goldsmith about the growing size and influence of UnitedHealth Group in the wake of the Change Healthcare hack.

    Plus, for “extra credit” the panelists suggest health policy stories they read this week that they think you should read, too: 

    Julie Rovner: Politico’s “Republicans Are Rushing to Defend IVF. The Anti-Abortion Movement Hopes to Change Their Minds,” by Megan Messerly and Alice Miranda Ollstein.

    Tami Luhby: The Washington Post’s “Biden Summons Bernie Sanders to Help Boost Drug-Price Campaign,” by Dan Diamond.

    Lauren Weber: The Washington Post’s “Bird Flu Detected in Dairy Worker Who Had Contact With Infected Cattle in Texas,” by Lena H. Sun and Rachel Roubein. 

    Joanne Kenen: The 19th’s “Survivors Sidelined: How Illinois’ Sexual Assault Survivor Law Allows Hospitals to Deny Care,” by Kate Martin, APM Reports.

    Also mentioned on this week’s podcast:

    Credits

    • Francis Ying Audio producer
    • Emmarie Huetteman Editor




    Kaiser Health NewsThis article was reprinted from khn.org, a national newsroom that produces in-depth journalism about health issues and is one of the core operating programs at KFF – the independent source for health policy research, polling, and journalism.



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  • WHO launches AI-powered empathetic digital health promoter

    WHO launches AI-powered empathetic digital health promoter

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    Ahead of World Health Day, focused on ‘My Health, My Right’, the World Health Organization (WHO) announces the launch of S.A.R.A.H., a digital health promoter prototype with enhanced empathetic response powered by generative artificial intelligence (AI).

    S.A.R.A.H. is a Smart AI Resource Assistant for Health that represents an evolution of AI-powered health information avatars, using new language models and cutting-edge technology. It can engage users 24 hours a day in 8 languages on multiple health topics, on any device.

    WHO’s digital health promoter is trained to provide information across major health topics, including healthy habits and mental health, to help people optimize their health and well-being journey. It aims to provide an additional tool for people to realize their rights to health, wherever they are.

    S.A.R.A.H., also known as Sarah, has the ability to support people in developing better understanding of risk factors for some of the leading causes of death in the world, including cancer, heart disease, lung disease, and diabetes. She can help people access up-to-date information on quitting tobacco, being active, eating a healthy diet, and de-stressing among other things. 

    The future of health is digital, and supporting countries to harness the power of digital technologies for health is a priority for WHO. S.A.R.A.H. gives us a glimpse of how artificial intelligence could be used in future to improve access to health information in a more interactive way. I call on the research community to help us continue to explore how this technology could narrow inequities and help people access up-to-date, reliable health information.”


    Dr. Tedros Adhanom Ghebreyesus, WHO Director-General 

    S.A.R.A.H. is now powered by generative AI rather than a pre-set algorithm or script helping her to provide more accurate responses in real-time; engage in dynamic personalized conversations at scale that more accurately mirror human interactions and provide nuanced, empathetic responses to users in a judgment-free environment. ​​The technology is supported by Soul Machines Biological AI. 

    WHO calls for continued research on this new technology to explore potential benefits to public health and to better understand the challenges. While AI has enormous potential to strengthen public health it also raises important ethical concerns, including equitable access, privacy, safety and accuracy, data protection, and bias.

    Continuous evaluation and refinement as part of this project emphasize WHO’s dedication to bringing health information closer to people while maintaining the highest standards of ethics and evidence-based content. Developers, policy makers and health care providers need to address these ethics and human rights issues when developing and deploying AI to ensure that all people can benefit from it.

    The S.A.R.A.H. project strives for continuous learning and development of a prototype that can inspire reliable, responsible and accessible information. 

    Previous iterations of S.A.R.A.H. were used to disseminate critical public health messages, under the name Florence, during the COVID-19 pandemic on the virus, vaccines, tobacco use, healthy eating and physical activity. 

    WHO continues to use many digitaltools and channels to disseminate and amplify health information including social media, chatbots, channels and text messaging.

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  • Unlocking clotting mechanisms in caterpillar hemolymph for medical use

    Unlocking clotting mechanisms in caterpillar hemolymph for medical use

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    Blood is a remarkable material: it must remain fluid inside blood vessels, yet clot as quickly as possible outside them, to stop bleeding. The chemical cascade that makes this possible is well understood for vertebrate blood. But hemolymph, the equivalent of blood in insects, has a very different composition, being notably lacking in red blood cells, hemoglobin, and platelets, and having amoeba-like cells called hemocytes instead of white blood cells for immune defense.

    The caterpillar of another species of moth, the yellow-striped oakworm (Anisota peigleri). Image credit: Konstantin Kornev
    The caterpillar of another species of moth, the yellow-striped oakworm (Anisota peigleri). Image Credit: Konstantin Kornev

    Just like blood, hemolymph clots quickly outside the body. How it does so has long remained an enigma. Now, materials scientists have shown in Frontiers in Soft Matter how this feat is managed by caterpillars of the Carolina sphinx moth. This discovery has potential applications for human medicine, the authors said.

    “Here we show that these caterpillars, called tobacco hornworms, can seal the wounds in a minute. They do that in two steps: first, in a few seconds, their thin, water-like hemolymph becomes ‘viscoelastic’ or slimy, and the dripping hemolymph retracts back to the wound,” said senior author Dr Konstantin Kornev, a professor at the Department of Materials Science and Engineering of Clemson University.

    “Next, hemocytes aggregate, starting from the wound surface and moving up to embrace the coating hemolymph film that eventually becomes a crust sealing the wound.”

    Challenging to study

    Fully grown tobacco hornworms, ready to pupate, are between 7.5cm and 10cm long. They only contain a minute amount of hemolymph, which typically clots within seconds, which makes it hard to study with conventional methods.

    For these reasons, Kornev and colleagues had to develop new techniques for the present study, and work fast. Even so, the failure rate for the trickiest manipulations was enormous (up to 95%), requiring many attempts.

    They restrained individual hornworms in a plastic sleeve, and made a slight wound in one of each caterpillar’s pseudolegs through a window in the sleeve. They then touched the dripping hemolymph with a metal ball, which was pulled away, creating a hemolymph ‘bridge’ (about two millimeters long and hundreds of micrometers wide) that subsequently narrowed and broke, producing satellite droplets. Kornev et al. filmed these events with a high frame rate camera and macro lens, to study them in detail.

    Instantaneous change in properties

    These observations suggested that during the first approximately five seconds after starting to flow, hemolymph behaved similarly to water: in technical terms, like a Newtonian, low viscosity liquid. But within the next 10 seconds, the hemolymph underwent a marked change: it now did not break instantaneously but formed a long bridge behind the falling drop. Typically, bleeding stopped completely after 60 to 90 seconds, after a crust formed over the wound.

    Kornev et al. studied the hemolymph’s flow properties further by placing a 10-micrometer-long nickel nanorod in a droplet of fresh hemolymph. When a rotating magnetic field caused the nanorod to spin, its lag relative to the magnetism gave an estimate of the hemolymph’s ability to hold the rod back through viscosity.

    They concluded that within seconds after leaving the body, caterpillar hemolymph changes from a low-viscous into a viscoelastic fluid.

    A good example of a viscoelastic fluid is saliva. When you smear a drop between your fingers, it behaves like water: materials scientists will say it is purely viscous. But thanks to very large molecules called mucins in it, saliva forms a bridge when you move your fingers apart. Therefore, it’s properly called viscoelastic: viscous when you shear it and elastic when you stretch it.”

    Dr Konstantin Kornev, Professor at Department of Materials Science and Engineering, Clemson University

    The scientists further used optical phase-contrast and polarized microscopy, X-ray imaging, and materials science modeling to study the cellular processes by which hemocytes aggregate to form a crust over a wound. They did this not only in Carolina sphinx moths and their caterpillars, but also in 18 other insect species.

    Hemocytes are key

    The results showed that hemolymph of all species studied reacted similarly to shear. But its reaction to stretching differed drastically between the hemocyte-rich hemolymph of caterpillars and cockroaches on the one hand, and the hemocyte-poor hemolymph of adult butterflies and moths on the other: droplets stretched out to form bridges for the first two, but immediately broke for the latter.

    “Turning hemolymph into a viscoelastic fluid appears to help caterpillars and cockroaches to stop any bleeding, by retracting dripping droplets back to the wound in a few seconds,” said Kornev. “We conclude that their hemolymph has an extraordinary ability to instantaneously change its material properties. Unlike silk-producing insects and spiders, which have a special organ for making fibers, these insects can make hemolymph filaments at any location upon wounding.”

    The scientists concluded that hemocytes play a key role in all these processes. But why caterpillars and cockroaches need more hemocytes than adult butterflies and moths is still unknown.

    “Our discoveries open the door for designing fast-working thickeners of human blood. We needn’t necessarily copy the exact biochemistry, but should focus on designing drugs that could turn blood into a viscoelastic material that stops bleeding. We hope that our findings will help to accomplish this task in the near future,” said Kornev.

    Source:

    Journal reference:

    Aprelev, P., et al. (2024) To seal a wound, caterpillars transform blood from a viscous to a viscoelastic fluid in a few seconds. Frontiers in Soft Matter. doi.org/10.3389/frsfm.2024.1341129.

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  • Study reveals e-cigarette users with limited smoking history show similar DNA alterations as smokers

    Study reveals e-cigarette users with limited smoking history show similar DNA alterations as smokers

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    A recent Cancer Research study assessed the effect of tobacco smoking and electronic cigarette (e-cigarettes) use on DNA methylation changes associated with carcinogenesis.

    Study: Cigarette smoking and e-cigarette use induce shared DNA methylation changes linked to carcinogenesis. Image Credit: Andrey_Popov/Shutterstock.com
    Study: Cigarette smoking and e-cigarette use induce shared DNA methylation changes linked to carcinogenesis. Image Credit: Andrey_Popov/Shutterstock.com

    Background

    In comparison to tobacco smoking, the use of e-cigarettes and smokeless, non-combustible tobacco has often been considered to be less harmful. However, recent studies have highlighted some of the potential adverse effects linked to e-cigarette use, including DNA damage and endothelial dysfunction. Therefore, it is imperative to understand the molecular changes and their long-term effects on health.

    E-cigarette use has been associated with similar biomarker changes to cigarette smoking. It is essential to identify biomarkers that indicate the risk of cancer. Some of the characteristic features that must be present in biomarkers are (a) modifiability through tobacco smoking and e-cigarette use, (b) lie in genes linked to carcinogenesis, (c) induce cancer progression in a premalignant lesion, and (d) reflective of long-term cancer risk in a surrogate tissue to aid non-invasive monitoring.

    The epigenome is a set of chemical modifications of DNA or proteins linked to DNA. Many studies have elucidated the role of epigenomics in carcinogenesis. This information has helped shed light on the long-term impacts of tobacco smoking and e-cigarette use.

    DNA methylation (DNAme) at the cytosine C-5 position is an epigenetic modification. Its alterations enriched in genes are associated with smoking-related diseases. Some epigenetic changes remain persistent even after smoking cessation. These biomarkers could be used as an indicator of lung cancer. 

    Epithelial cells that are exposed (e.g., oral mucosa and lungs) or not (e.g., cervix) to smoking or e-cigarette use are the key cells of origin for tobacco-related malignancies. Furthermore, smoking-related DNAme changes found in buccal samples are primarily of epithelial origin.

    About the study

    The current study addressed the aforementioned issues to understand better tissue- and cell-specific epigenetic impacts of e-cigarette or tobacco use on DNAme. It used more than 3,500 cervical, buccal/saliva, or blood samples from immune and epithelial cells at directly and indirectly exposed sites. Additionally, a control sample set was used for validation.

    This study is a part of the female cancer prediction using cervical omics to individualize screening and prevention (FORECEE) study. The participants came from five European countries, were between 18 and 86 years of age, and completed an epidemiological questionnaire. The effect of tobacco use on epithelial and immune cells was analyzed systematically using computational deconvolution and cell type-specific DNAme inference.

    The effect of direct (proximal) and indirect (distal) exposure to the use of smoking, smokeless tobacco (e.g., snuffs), or e-cigarettes on epithelial and immune cells was assessed. Furthermore, whether these uses also affected lung cancer tissue and prognosis were evaluated. The evaluation of the biomarkers at the cell-level is a key contribution of this study, as the majority of existing studies, including those predicting lung cancer, have used blood samples.

    Study findings

    The cell-specific alterations following cigarette and e-cigarette use that are associated with carcinogenesis have been uncovered in this study. Smoking was found to elicit changes in protected stem and submucosal gland cells. Cigarette smoking affected epithelial hypoM and this change was found in both proximal and distal exposure. Furthermore, DNAme alterations linked to specific proximal epithelial hyperM and distal epithelial hyperM were also identified.

    Smoking was seen to affect the myeloid more prominently than the lymphoid lineage. No significant genetic overlap linked with specific functions was observed in the samples obtained from five different sites. Mostly, epithelial hypoM sites were linked with detoxification responses, whereas proximal epithelial hyperM sites entailed DNA damage responses.

    The smoking-related DNAme loci (CpGs) identified here were clustered into four functional group based on anatomical site and cell type. Loci hypermethylated in cheek cells of smokers associated with the NOTCH1/RUNX3/growth factor receptor signaling showed a higher level of methylation in progressing lung carcinoma in situ lesions and cancer tissue. Alarmingly, the aforementioned CpGs were also noted to be hypermethylated in e-cigarette users who reported a limited smoking history.

    This study further highlighted a partial reversibility of smoking-induced epigenetic alterations in former tobacco smokers. This observation was based on the fact that epithelial hypoM could not be distinguished between ex-smokers and those who never smoked. This observation could imply that the hypermethylated cells disappeared due to cell death or the displacement of the methyl group in the living cell.

    Smokeless tobacco induces similar changes in DNAme in the epithelial hypoM and proximal epithelial hyperM sets, as cigarette smoking. It must be noted that only cigarette smokers exhibited changes in DNAme at immune hypoM sites. Proximal epithelial hypermethylation was robustly associated with lung cancer progression and cervical cancer.

    Conclusions

    In sum, the results presented here shed light on cell type-specific epigenetic changes following cigarette smoking. Some of these changes, which could also predict lung cancer, are similar to e-cigarette users.

    A key limitation of this study was the use of pathway analysis based on gene names, which limited the investigation to cis genes alone. In the future, scientists must perform multi-omics profiling to investigate the association between methylation changes and gene transcription function more comprehensively. 

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  • Second-hand vape smoke linked to more asthma symptoms in kids

    Second-hand vape smoke linked to more asthma symptoms in kids

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    In a recent study published in the journal Children, researchers retrospectively investigated the impacts of second-hand e-cigarette smoke exposure on childhood asthma, especially in home environments. They carried out a pilot, monocenter, observational study of 54 young asthma patients, half of whom experienced second-hand exposure (SHE) to second-hand aerosols (SHAs).

    Study: Association between Second-Hand Exposure to E-Cigarettes at Home and Exacerbations in Children with Asthma. Image Credit: Prostock-studio / ShutterstockStudy: Association between Second-Hand Exposure to E-Cigarettes at Home and Exacerbations in Children with Asthma. Image Credit: Prostock-studio / Shutterstock

    Despite finding no statistically significant association between electronic nicotine delivery systems (ENDSs) and asthma exacerbations (no difference in the number of patients requiring clinical intervention step-up), this study suggests that asthmatic children exposed to elevated levels of second-hand e-cigarette smoke may experience increases in their number of asthma symptomatic days. This highlights the need for heightened awareness, both amongst adolescents and their parents, of the psychological harms of the ‘safe’ vape.

    The ENDs pandemic and what this means for asthma patients

    Extensive research and medical reports highlight tobacco smoking as the single most preventable cause of global mortality and morbidity, with the habit associated with significant increases in the risks of numerous cancers, cardiovascular diseases (CVDs), respiratory ailments, and psychiatric disorders. Long-term global efforts have resulted in substantial reductions in tobacco use prevalence amongst adults and adolescents, representing one of the most noteworthy accomplishments of modern public health.

    Unfortunately, in recent years, tobacco smoking has been replaced by the use of electronic nicotine delivery systems (ENDSs). Commonly called ‘vapes,’ these devices are marketed as low- or no-risk alternatives to conventional smoking. While they are devoid of tar and a majority of the heavy metal components that make tobacco smoke harmful, recent research presents a growing body of evidence suggesting that ENDs are not as safe as we may think. Even non-smokers who take up vaping have been shown to develop adverse and often chronic respiratory symptoms, including bronchoconstriction and severe cough.

    Asthma is a respiratory condition characterized by difficulty breathing, chest pain, cough, and wheezing, which in severe cases may lead to life-threatening suffocation. Caused by the inflammation or narrowing of a patient’s airways or excessive mucus secretions along the respiratory tract, the condition is most common in young children. It presents the most common pediatric disease worldwide. Unfortunately, while a few studies have investigated the associations between e-cigarette exposure and asthma in adults and found that the former can exacerbate the latter, the impacts of second-hand exposure on pediatric asthma have hitherto remained unexplored.

    “Establishing evidence of adverse health effects caused by second-hand nicotine vaping exposure could represent a valid motivation for minimizing household exposure and imposing restrictions on vaping in public spaces.”

    About the study

    Aerosols produced by ENDs are known to contain volatile aldehydes and oxidant metals, some of which have been shown to produce adverse outcomes in adult patients’ lungs, both asthmatic and non-asthmatic. Unfortunately, the effects of these volatile organic compounds (VOCs) on children’s lungs remain unknown. The present study aims to fill this knowledge gap by retrospectively elucidating the associations between childhood ENDs exposure and asthma symptom progression.

    The observational study was carried out between January and May 2023 at “Gaetano Martino” Hospital, University of Messina, Italy, and comprised children or adolescents aged five to 17 with medically confirmed asthma. Data collection included demographics (age, sex, gender, and race), clinical (comorbidities), parents’ socioeconomic status, and the education levels of both parents and children. Additionally, ENDs exposure was recorded in terms of presence (yes/no) and frequency. All data was collected using a custom-designed questionnaire.

    The Asthma Control Test (ACT) and the children-Asthma Control Test (c-ACT were administered at the time of initial study enrolment. Patients were assigned to asthma or no-asthma cohorts (n = 27 per cohort), with analyses stratified to account for age – two age cohorts (5-11 [n = 65%] and 12-17 [n = 35%]). Continuous data variables were analyzed using descriptive statistics (expressed as means and standard deviations [SDs]), while ordinary variables were expressed as percentages. Fisher’s tests were used to compare cohorts qualitatively, while independent t-tests computed differences between continuous variables across cohorts.

    Study findings and conclusions

    The total sample size for the present study was 54, equally divided between children whose parents indulge in e-cigarette consumption at home and those whose parents do not. Of these, 39 were diagnosed with intermittent, nine with moderate, and six with severe asthma, respectively.

    While the dataset was too small to provide statistically significant differences between asthma and non-asthma cohorts, descriptive statistics reveal that ENDs had more profound impacts on younger children (Group A – 5 to 11 years) compared to their older counterparts (Group B – 12 to 17 years) with the former group needing six times more rescue therapy and 15% more therapeutic step-up than the former. These results are in concordance with the conventional assumption that younger children are at higher risk of asthma contraction due to their undeveloped immune systems and narrower respiratory passages.

    Despite not yielding statistically significant results, the proceeds of this study highlight the risk posed by household END usage to children. While not as harmful as conventional tobacco smoke, vaporization of e-liquids is known to release significant qualities of aldehydes, including formaldehyde, known for being respiratory irritants and carcinogens. Previous research comparing harmful aerosol concentrations in home environments raises cause for concern – ultrafine particulate matter produced by e-cigarettes matches. It sometimes exceeds that produced by an equivalent amount of tobacco smoke.

    “…our data highlight the importance of the prevention of the vaping epidemic and passive exposure to e-cigarettes, even among children and adolescents. Implementing educational programs to increase awareness about the risks of vaping among children and emphasizing the potential impact on respiratory health, especially for those with asthma, should be a priority. Launching targeted campaigns to inform parents about the dangers of vaping and its specific implications for children with asthma should be strengthened.”

    Journal reference:

    • Costantino, S., Torre, A., Foti Randazzese, S., Mollica, S. A., Motta, F., Busceti, D., Ferrante, F., Caminiti, L., Crisafulli, G., & Manti, S. (2024). Association between Second-Hand Exposure to E-Cigarettes at Home and Exacerbations in Children with Asthma. Children, 11(3), 356, DOI – 10.3390/children11030356,  https://www.mdpi.com/2227-9067/11/3/356

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  • E-cigarette users experience similar DNA changes to specific cheek cells as smokers

    E-cigarette users experience similar DNA changes to specific cheek cells as smokers

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    E-cigarette users with a limited smoking history experience similar DNA changes to specific cheek cells as smokers, finds a new study led by researchers at UCL (University College London) and University of Innsbruck.

    This study is an incremental step in helping researchers to build a deeper understanding of the long-term effects of e-cigarettes on health. Although it does not show that e-cigarettes cause cancer, studies with long-term follow up are important to assess whether e-cigarettes have harmful effects and, if so, what they are.

    The study, published in Cancer Research, analysed the epigenetic effects of tobacco and e-cigarettes on DNA methylation in over 3,500 samples, to investigate the impact on cells that are directly exposed to tobacco (e.g. in the mouth) and those that are not directly exposed (e.g. in blood or cervical cells).

    The epigenome refers to an extra layer of information that is superimposed on our genetic material – the DNA. While DNA can be compared to the ‘hardware’ of a computer, epigenetics are comparable to the computer’s ‘software’ and define how, where and when the programs used by the computer are run.

    Epigenomes change throughout our lives and can be affected by a variety of genetic or nongenetic factors – including aging, our lifestyles, exposure to hormones, chemicals and environmental factors, and even stress and psychological trauma. One commonly studied type of epigenetic modification is called DNA methylation.

    The researchers found that epithelial cells (cells that typically line organs and are often the cells of origin for cancer) in the mouth showed substantial epigenomic changes in smokers. Importantly, these changes are further elevated in lung cancers or pre-cancers (abnormal cells or tissue that have the potential to develop into cancer), when compared to the normal lung tissue, supporting the idea that the epigenetic changes associated with smoking allow cells to grow more quickly.

    The publication also includes new data showing the similar epigenomic changes were likewise observed in the cells of e-cigarette users who had only ever smoked less than 100 tobacco cigarettes in their lives.

    First author, Dr Chiara Herzog (UCL EGA Institute for Women’s Health and University of Innsbruck), said: “This is the first study to investigate the impact of smoking and vaping on different kinds of cells – rather than just blood – and we’ve also strived to consider the longer-term health implications of using e-cigarettes.

    “We cannot say that e-cigarettes cause cancer based on our study, but we do observe e-cigarette users exhibit some similar epigenetic changes in buccal cells as smokers, and these changes are associated with future lung cancer development in smokers. Further studies will be required to investigate whether these features could be used to individually predict cancer in smokers and e-cigarette users.

    “While the scientific consensus is that e-cigarettes are safer than smoking tobacco, we cannot assume they are completely safe to use and it is important to explore their potential long-term risks and links to cancer.

    “We hope this study may help form part of a wider discussion into e-cigarette usage – especially in people who have never previously smoked tobacco.”

    Through their computational analysis of the samples, the researchers also found that some smoking-related epigenetic changes remain more stable than others after giving up smoking, including smoking-related epigenetic changes in cervical samples – something that has not previously been studied.

    Senior author, Professor Martin Widschwendter (UCL EGA Institute for Women’s Health and University of Innsbruck), said: “The epigenome allows us, on one side, to look back. It tells us about how our body responded to a previous environmental exposure like smoking. Likewise exploring the epigenome may also enable us to predict future health and disease. Changes that are observed in lung cancer tissue can also be measured in cheek cells from smokers who have not (yet) developed a cancer. Importantly, our research points to the fact that e-cigarette users exhibit the same changes, and these devices might not be as harmless as originally thought. Long-term studies of e-cigarettes are needed. We are grateful for the support the European Commission has provided to obtain these data.”

    Tobacco is well known as a modifiable contributor to adverse health outcomes, and it has been estimated to have caused 7.69 million deaths globally in 2019, with numbers expected to increase in the future. The NHS says e-cigarettes are substantially safer than smoking tobacco and smokers are recommended to switch to vaping to improve their health.

    The researchers involved in the latest study now hope to further investigate how epigenetic changes related to smoking in cheek swabs could be used for identifying individuals at highest risk of developing cancer and assess the long-term health risks of e-cigarettes. 

    This work was supported by funding from the European Union’s Horizon 2020 Research and Innovation programme, The Eve Appeal, and Cancer Research UK.

    This study contributes to our understanding of e-cigarettes, but it does not show that e-cigarettes cause cancer. Decades of research has proven the link between smoking and cancer, and studies have so far shown that e-cigarettes are far less harmful than smoking and can help people quit. This paper does however highlight that e-cigarettes are not risk-free, and so we need additional studies to uncover their potential longer-term impacts on human health.


    Smoking tobacco causes 150 cases of cancer every single day in the UK, which is why we look forward to seeing the Government’s age of sale legislation being presented in parliament. Nothing would have a bigger impact on reducing the number of preventable deaths in the UK than ending smoking, and this policy will take us one step closer to a smokefree future.”


    Dr Ian Walker, Cancer Research UK’s executive director of policy

    Source:

    Journal reference:

    Herzog, C., et al. (2024) Cigarette smoking and e-cigarette use induce shared DNA methylation changes linked to carcinogenesis. Cancer Research. doi.org/10.1158/0008-5472.CAN-23-2957.

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  • Lower grades for students who use both tobacco and cannabis, California survey reveals

    Lower grades for students who use both tobacco and cannabis, California survey reveals

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    Several reports have raised the issue of increasing absenteeism and lower grades in American public schools after they reopened following the coronavirus disease 2019 (COVID-19) pandemic. These predict higher rates of mental illness and dropout from high school. Substance use is also linked to poor outcomes, including vaping, tobacco, and cannabis, perhaps because of their effects on the developing brain.

    Study: Co-Use of Tobacco Products and Cannabis Is Associated with Absenteeism and Lower Grades in California High School Students. Image Credit: Solid photos/Shutterstock.com
    Study: Co-Use of Tobacco Products and Cannabis Is Associated with Absenteeism and Lower Grades in California High School Students. Image Credit: Solid photos/Shutterstock.com

    A recent study published in The Journal of Pediatrics examines the odds of these outcomes when tobacco and cannabis are co-used after compensating for other risks. With the rise in legalized cannabis, there has been an increase in the national (but not California) proportion of young people who vape, from 11% to 21% over the period 2017 to 2022. Again, the proportion of cannabis users went up from 5% to 15%.

    The current study sought to dissociate this risk in a sample of high school students in California surveyed in 2020-21, immediately after post-COVID school reopening.

    About the study

    The study utilized the California Healthy Kids Survey (CHKS), performed by the WestEd, the California Department of Education, and the Department of Health Care Services, on students in grades 9 and 11 in California. Only public school students were included, numbering about 353,000, who responded to survey questions about tobacco and cannabis use during the 30 days just before the survey and were attending school in person.

    The sample was equally split between males and females, grades 9 and 11, and those with highly educated parents vs others. Almost half were Hispanic, a quarter non-Hispanic White, and 15% Asian. Over one in three said they had felt depressed sometime in the past year, while 6% reported feeling endangered at school.

    Almost a tenth used alcohol, while 2% used tobacco alone. About 4% used cannabis, and the same proportion used both substances. Almost double these numbers were reported as having used these substances at any time.

    Co-use of both substances was linked to 35% absenteeism vs 29% in cannabis-only users, 26% among tobacco-only users, and 17% among those who used neither. Risk factors for absenteeism and/or poor grades that needed to be adjusted for included bullying, poor health, and change of residence if the student is a caregiver for others or comes from a family with food insecurity. Unsafe school conditions or poor educational climates at school also play a role in this phenomenon.

    After adjustment, co-users had a 40% higher risk of absenteeism, the highest among all categories. Compared to tobacco-only users, co-use and cannabis-only users both were ~20% and 15% more likely to report absenteeism, respectively. Similar findings were obtained among ever-users, too.

    The mean grade was 6.16, that is, mostly Bs. Co-use was associated with a mean grade of 5.08 vs 5.61 for tobacco-only users, 5.54 for cannabis-only users, and 6.24 among non-users. If using tobacco only was the reference group, co-users, and cannabis-only users had a decrease of 0.39 points, but it fell by a mean of 0.87 points when co-users were matched to non-users. Cannabis-only and tobacco-only users had no difference in their mean grades.

    Dangers of absenteeism

    School funding suffers when students are absent. Thus, co-use has roughly cost the school $300 per absent student on average.

    With over 8,000 students reporting substance use, absences may potentially have cost the school almost $2.5 million in annual funding, provided all of these absences were due to substance use, though this is an unlikely event.

    What are the implications?

    The drop in grades by almost one category, from Bs to Bs and Cs, associated with co-users compared to non-users, is an important adverse effect. This was accompanied by 40% higher odds of absenteeism (being absent three or more days within the past month) in the co-user group vs non-users and 20% higher odds than among tobacco-only users.

    This novel study supports the hypothesis that substance abuse, especially the dual use of tobacco and alcohol, worsens educational outcomes. Several mechanisms have been speculated about. For instance, these substances could affect cognitive processing and disrupt learning and memory pathways.

    Cannabis heightens nicotine addiction when used during adolescence, making quitting very difficult. Also, the use of either or both of these substances can cause illness, physical or mental, resulting in skipping school. Co-use has been linked to changes in the sleep pattern.

    Finally, vaping at school may distract students, reducing their ability to learn. Another possibility is that vaping or co-use may result in suspension from school. The occurrence of such events is mirrored in the list of reasons for absenteeism, such as illness, anger, sadness, stress, or inadequate sleep.

    Future studies should assess potential interventions to improve educational outcomes with the help of full-spectrum efforts to reduce or eliminate substance use among students.

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  • Lesbian, gay and bisexual women respond more positively to tobacco marketing

    Lesbian, gay and bisexual women respond more positively to tobacco marketing

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    People who identify as lesbian, gay and bisexual – particularly women – respond more positively to tobacco marketing, are more inclined to smoke cigarettes daily and may have a more difficult time quitting, according to two studies by a Rutgers Health researcher.

    The studies, published in the Annals of LGBTQ Public and Population Health and Preventive Medicine Reports, investigated how some among the LGBTQ population respond to tobacco marketing, how they use tobacco and their history of quitting using two large national datasets, including the Population Assessment of Tobacco and Health Study and the National Survey on Drug Use and Health.

    Ollie Ganz, a faculty member at the Rutgers Institute for Nicotine and Tobacco Studies and an assistant professor at the Rutgers School of Public Health who is lead author of the studies, discussed the significance of the findings to future policy.

    Why is specifically studying how sexual minority populations receive tobacco marketing important?

    From previous studies, we know that sexual minority individuals are more likely to use tobacco products than heterosexual individuals. This may be because tobacco companies have targeted this population with advertising and promotions, but the impact of this has not been studied extensively. The main thing we wanted to understand in this study is, given that sexual minority individuals are exposed to more tobacco advertising than heterosexual individuals, are there also differences in terms of how receptive they are to the ads, such as having a positive response to the ads.

    What did your study find regarding tobacco marketing among sexual minority individuals?

    What was unique about our study is that we were able to look at subsets of the LGB population – gay men, bisexual men, lesbian/gay women and bisexual women – rather than looking at them as one group. By looking at these subgroups we discovered that lesbian/gay and bisexual women were more likely to be receptive to marketing for tobacco products overall, and that bisexual women – but not lesbian/gay women – were more likely to be receptive to marketing for cigarettes, cigars, e-cigarettes and smokeless tobacco, compared with heterosexual women.

    Among men, we discovered that gay men, but not bisexual men, were more likely to be receptive to cigar advertising compared with heterosexual men. We also found that gay and bisexual men were more likely to be receptive to e-cigarette advertising compared with heterosexual men.

    In the Preventive Medicine Reports study, you also looked at subsets of the LGB population, but focused on cigarette use behaviors. What were your findings?

    We discovered that sexual minority women – particularly bisexual women – are smoking at higher rates and are having a harder time quitting compared with heterosexual women. We also found that the differences in prevalence of cigarette smoking between lesbian/gay and bisexual women and heterosexual women was much greater than between the prevalence between sexual minority men and heterosexual men.

    These findings show that we need more support to specifically help sexual minority women quit smoking –particularly bisexual women. This population presents unique challenges, such as greater mental health problems, and there is a need for more resources and tailored interventions to support them in quitting smoking.

    How can these findings inform future policy?

    In order for tobacco control policies and regulations and public education campaigns to be effective, they need to be able to identify the populations that are at greatest risk for tobacco use. They also need a body of evidence that identifies the factors that are driving elevated use among those populations so effective messages and policies can be developed that directly address those factors.

    Source:

    Journal references:

    • Ganz, O., et al. (2024) Differences in Tobacco Advertising Receptivity Among Young Adults by Sexual Identity and Sex: Findings From the Population Assessment of Tobacco and Health Study. Annals of LGBTQ Public and Population Health. doi.org/10.1891/LGBTQ-2022-0036.
    • Ganz, O., et al. (2024). Cigarette Smoking Behaviors and Nicotine Dependence at the Intersection of Sexual Identity and Sex in the United States: Findings from the National Survey on Drug Use and Health. Preventive Medicine Reports. doi.org/10.1016/j.pmedr.2024.102593.

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