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Nature, Published online: 11 June 2024; doi:10.1038/d41586-024-01700-6
Buried items show that the poor got poorer as the Assyrian empire and its bureaucracy swelled.
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The domestication of horses began on the Eurasian steppes
Lina Shatalova/iStockphoto/Getty Images
Ancient breeders dramatically shortened the natural generation times of horses starting about 4200 years ago, according to a genetic study of hundreds of ancient horses. This intensive breeding led to a massive expansion of those bloodlines across Eurasia within a few centuries, says Ludovic Orlando at the Centre for Anthropobiology and Genomics of Toulouse, France.
“In other words, they controlled the reproduction of the horse,” he says. “So this tells us something about the process of breeding that was underlying the success of the expansion of horses around the world.”
Horses were first domesticated 5500 years ago by the Botaï people in what is now Kazakhstan, but they didn’t spread their horse culture elsewhere, says Orlando. The Botaï eventually died out and their horses returned to the wild.
More than a thousand years later, however, a different line of horses became domesticated in the Pontic-Caspian steppes of southern Russia. It was this line that ultimately spread across the planet, leading to every domestic horse in the world today, he says.
To chart the history of horse husbandry, Orlando and his colleagues analysed the genomes of 475 ancient horses from Eurasia dating up to 50,000 years ago. They compared those with the genomes of 71 modern domestic horses representing 40 breeds worldwide, as well as six endangered Przewalski’s horses – which are a different sub-species.
The team confirmed that horses prior to the third millennium BC weren’t being bred or domesticated – except among the Botaï. This means horses didn’t contribute to human migrations and cultural expansions before that time, contrary to some theories, says Orlando.
The DNA analysis revealed significant inbreeding 4200 years ago in the Pontic-Caspian steppe horses, probably because people aimed to develop specific traits that make high-quality riding and chariot horses, he says.
Then, using a new technique combining genome sequencing and carbon dating, the scientists were able to estimate the average number of years between two successive generations, which Orlando calls the generational time interval. That interval got remarkably shorter – half as long as in the wild – during the same period of massive inbreeding in the Pontic-Caspian steppes.
“Right at the time of the domestication bottleneck, around 2200 BC, this is when breeders managed to control the reproduction of the horse so much that generations were ticking faster and faster,” says Orlando.
Orlando suspects the breeders were probably shortening generations by having them mate at younger ages than they would in the wild, he said at the International Havemeyer Foundation Horse Genome Workshop, which took place last month in Caen, France.
Christine Aurich at the University of Veterinary Medicine Vienna suspects the shortened generations were probably due to better survival rates rather than younger breeding ages. Horses give birth lying down in open grasslands, making them highly susceptible to predators until the foal can run, several hours later. Plus, any disturbances could prevent the foal from drinking its first milk – which always leads to death.
“It must be assumed that for horses living in the care of humans, losses of mares and their newborn foals were considerably reduced in comparison to horses living under wildlife conditions,” says Aurich.
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The skull of an ancient Egyptian man with a round lesion caused by a tumour near the top
Tondini, Isidro, Camarós, 2024
Tiny etchings on a 4000-year-old skull suggest that the ancient Egyptians may have been the first people to try to treat cancer.
“Ancient Egyptian medicine was very advanced,” says Edgard Camarós at the University of Santiago de Compostela in Spain. “They were able to deal with many conditions, including traumatisms and infections, and treat oral health.”
Camarós and his…
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The Red Pyramid at Dahshur in Egypt was one of many built close to a lost branch of the Nile
Eman Ghoneim
Many of the pyramids of ancient Egypt were built along a now extinct branch of the river Nile, geological surveys have revealed. This could explain why these pyramids, including the famed Great Pyramid of Giza, are clustered in a thin strip of arid, inhospitable land.
“Since ancient times, the Nile has provided sustenance to Egyptian settlements, and it functions as the main water corridor that allowed for the transportation of goods and building materials in the past,” says Tim Ralph at Macquarie University in Sydney, Australia. “For this reason, most of the key cities and monuments were built in close proximity to the banks of the Nile and its peripheral branches.”
More than 100 pyramids were constructed between 4700 and 3500 years ago as grand tombs for Egypt’s pharaohs. Thirty-one of these, including the pyramids of Dahshur, Giza and Saqqara, are dotted along the edge of Egypt’s Western desert, several kilometres away from the Nile.
To transport the enormous number of people and resources necessary to build these pyramids, researchers have long thought that the Nile may have once had an offshoot that flowed by the construction sites.
To investigate further, Ralph and his colleagues looked at radar satellite imagery and land elevation data of the region. Depressions in the landscape indicated that the old water channel may have stretched 64 kilometres past the pyramid fields between the northern city of Giza and the village of Lisht in the south. It was also close to the ancient Egyptian capital of Memphis and the Abusir, Saqqara and Dahshur pyramid complexes.
Once the researchers had a rough idea of the branch’s location, they took soil and sediment core samples along its path and discovered a riverbed of sand hidden under what is now farmland or desert.
“We guess that it was roughly between 200 and 700 metres wide, and at least 8 metres deep at its deepest,” says Ralph.
Causeways that have been found around the 31 pyramids seem to end at the banks of this ancient Nile branch – a sign that the water channel was used to transport building materials thousands of years ago.
The ancient offshoot, dubbed the Ahramat branch after the Arabic word for pyramid, eventually dried up after a severe drought hit the region around 4200 years ago, says Ralph.

The course of the ancient Ahramat branch of the Nile
Eman Ghoneim et al.
“The existence of the channel is an excellent result,” says Penny Wilson at Durham University, UK. “Mapping all of this is a wonderful addition to the ancient landscape that has been buried and shows a cost-efficient way to reconstruct and re-evaluate the economic and social systems of the pharaonic state.”
Campbell Price at the University of Liverpool, UK, says: “I think people often imagine Egyptian pyramids being marooned in the middle of the desert.”
“This research seems to further demonstrate that they were in fact closely connected with the agricultural life of pharaonic Egypt – and the river Nile,” he says.
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