Browsing Tag
Cryoelectron microscopy
54 posts
Crosslinking intermodular condensation in non-ribosomal peptide biosynthesis
Felnagle, E. A. et al. Nonribosomal peptide synthetases involved in the production of medically relevant natural products. Mol.…
Native DGC structure rationalizes muscular dystrophy-causing mutations
Hoffman, E. P., Brown, R. H. Jr. & Kunkel, L. M. Dystrophin: the protein product of the Duchenne…
Structure of apolipoprotein B100 bound to the low-density lipoprotein receptor
Segrest, J. P., Jones, M. K., De Loof, H. & Dashti, N. Structure of apolipoprotein B-100 in low…
Structure and assembly of the dystrophin glycoprotein complex
Campbell, K. P. & Kahl, S. D. Association of dystrophin and an integral membrane glycoprotein. Nature 338, 259–262…
MCM double hexamer loading visualized with human proteins
Protein sequences Human cDNAs were cloned from RPE-1 and U2OS cells by total RNA isolation, oligo-dT reverse transcription,…
Multiple mechanisms for licensing human replication origins
Bleichert, F. Mechanisms of replication origin licensing: a structural perspective. Curr. Opin. Struct. Biol. 59, 195–204 (2019). Article …
Read–write mechanisms of H2A ubiquitination by Polycomb repressive complex 1
Stewart-Morgan, K. R., Petryk, N. & Groth, A. Chromatin replication and epigenetic cell memory. Nat. Cell Biol. 22,…
Nucleosome flipping drives kinetic proofreading and processivity by SWR1
Purification of wild-type SWR1 Recombinant SWR1 was produced as previously described12,16 with minor modifications. Baculoviruses encoding SWR1 genes…
Engineered odorant receptors illuminate the basis of odour discrimination
Buck, L. & Axel, R. A novel multigene family may encode odorant receptors: a molecular basis for odor…
LYCHOS is a human hybrid of a plant-like PIN transporter and a GPCR
Protein expression and purification The gene encoding LYCHOS (GPR155) (Uniprot Q7Z3F1), was synthesized and subcloned into pTwist CMV…