Browsing Tag
Protein design
9 posts
Synthetic GPCRs for programmable sensing and control of cell behaviour
Plasmid constructs and cloning Constructs used for transient expression in HEK293T cells were cloned into the pAAV viral…
Design of customized coronavirus receptors
Millet, J. K., Jaimes, J. A. & Whittaker, G. R. Molecular diversity of coronavirus host cell entry receptors.…
Transferrin receptor targeting chimeras for membrane protein degradation
Mayle, K. M., Le, A. M. & Kamei, D. T. The intracellular trafficking pathway of transferrin. Biochim. Biophys.…
Designed endocytosis-inducing proteins degrade targets and amplify signals
Computational design of sortilin minibinders as Sort_EndoTags Using a Rosetta-based binder design protocol15, 21,000 binders were generated to…
Computational design of soluble and functional membrane protein analogues
AF2seq design protocol Design target preparation The design target structures were sourced from the Protein Data Bank (PDB)…
Selective haematological cancer eradication with preserved haematopoiesis
Structural dataset and computational analysis The experimentally determined 3D structure of the CD45 extracellular domain was retrieved from…
Computationally restoring the potency of a clinical antibody against Omicron
Antibody and antigen production We experimentally validated the 376 designed candidates. To leverage available resources at multiple experimental…
Emergence of fractal geometries in the evolution of a metabolic enzyme
Molecular cloning The gene encoding CS from S. elongatus PCC 7942 was amplified from genomic DNA by PCR (Q5…
Blueprinting extendable nanomaterials with standardized protein blocks
Natural and previously designed proteins exhibit a wide range of helical geometries with local irregularities, kinks and deviations…