Browsing Tag
Single-molecule biophysics
6 posts
The mechanism of mRNA cap recognition
Gingras, A. C., Raught, B. & Sonenberg, N. eIF4 initiation factors: effectors of mRNA recruitment to ribosomes and…
Tracking transcription–translation coupling in real time
Strains and plasmids Ribosome mutant SQ380 strains (B68 strain, 30S helical extension in h33a; C68 strain, 30S helical…
Nucleosome flipping drives kinetic proofreading and processivity by SWR1
Purification of wild-type SWR1 Recombinant SWR1 was produced as previously described12,16 with minor modifications. Baculoviruses encoding SWR1 genes…
Multi-pass, single-molecule nanopore reading of long protein strands
Expression and purification of proteins Plasmids for analyte proteins were constructed using gBlocks (Integrated DNA Technologies) inserted into…
FANCD2–FANCI surveys DNA and recognizes double- to single-stranded junctions
Cloning and protein purification A codon-optimized construct of full-length Gallus gallus FANCD2 including a carboxyl-terminal double StrepII tag…
Stepwise activation of a metabotropic glutamate receptor
Pin, J.-P. & Bettler, B. Organization and functions of mGlu and GABAB receptor complexes. Nature 540, 60–68 (2016).…