Browsing Tag
Targeted gene repair
2 posts
Structural basis for pegRNA-guided reverse transcription by a prime editor
Sample preparation The PE2 (nSpCas9–engineered M-MLV RT), Cas9 (H840A) and M-MLV RTΔRNaseH (D200N/T306K/W313F/T330P) genes were PCR-amplified from pCMV-PE2…
May 29, 2024
Durable and efficient gene silencing in vivo by hit-and-run epigenome editing
Molecular cloning and mRNA production ETRs were transiently delivered as either plasmid DNA (in cell lines) or mRNA…
February 28, 2024