Browsing Tag
Cryoelectron tomography
4 posts
In situ analysis reveals the TRiC duty cycle and PDCD5 as an open-state cofactor
Cell culture HEK (HEK Flp-In T-Rex 293, Invitrogen) cells were cultured in DMEM (Sigma-Aldrich) supplemented with 10% fetal…
Visualizing chaperonin function in situ by cryo-electron tomography
Plasmids and strains Escherichia coli BL21(DE3) Gold cells (Stratagene) were used for growth analysis, electron tomography and protein…
Membraneless channels sieve cations in ammonia-oxidizing marine archaea
Könneke, M. et al. Isolation of an autotrophic ammonia-oxidizing marine archaeon. Nature 437, 543–546 (2005). Article ADS PubMed …
Bridging structural and cell biology with cryo-electron microscopy
Berman, H., Henrick, K. & Nakamura, H. Announcing the worldwide Protein Data Bank. Nat. Struct. Biol. 10, 980…